Prime Research on Biotechnology

Volume 1, Issue 1, pp. 01-05

Full Length Research

Isolation and identification of probiotic strains from different commercial products of Lahore, Pakistan

Ammara Hassan¹*, I. Amjad², M. Nawaz Chaudhry³, and Ifftiqar Bloach³

¹*Food & Biotechnology Research Centre, PCSIR Labs. Complex Lahore

²Haleeb Foods Pvt Limited, Lahore

³College of Earth & Environmental sciences, University of the Punjab, Lahore

*Corresponding author. E-mail: ammara.pcsir@gmail.com

Accepted 20^th May, 2011

In this study, ten commercially available probiotic products were collected; from market of Lahore, Pakistan; including dairy, freeze dried products and fruit drinks. These products are examined by using different media as well as by visualization of DGGE band profiles under UV light. Identical results of the DGGE and culurae-dependent analysis of the 10 probiotic products were found. But DGGE analysis of five products (Nestle yogurt, Haleeb Flavoured yogurt, Haleeb Yoghurt, Freeze-dried product, and Nestle fruit yogurt) detected the same species that were detected with conventional isolation procedures. For the remaining five products (Nestle nido, Haleeb Xtra energy milk, Haleeb Labban, Haleeb chadder cheese, and Freeze-dried culture), DGGE analysis was able to detect more claimed species than were recovered by isolation and for freeze-dried culture; species other than those mentioned on the label were isolated and identified. In this study, analysis of type strains representing known probiotic species never resulted in two species with identical band positions. These isolated stains of probiotics can be used in improving intestinal microbial balance and in alleviation of chronic intestinal inflammatory diseases, prevention and treatment of pathogen-induced diarrhea, urogenital infections, and atopic diseases.

Key words: Probiotic, diseases, pH, Lahore, Pakistan
 

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