E-test antibiotic susceptibility of Staphylococcus aureus strains isolated from hospital acquired infections of Imam Khomeini hospital, Ilam, Iran

Iraj Pakzad¹, Nourkhoda Sadeghifard¹, Farid Azizi Jalilian¹, Hassan Hossainzadegan*² , Sobhan Ghafouryan¹

¹Department of Microbiology and Clinical Microbiology Research Center, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran

²Department of Microbiology, Faculty of Nursing and Midwifery, Maraghah, Tabriz University of Medical Sciences, Tabriz, Iran

Accepted 29^th January, 2012

Staphylococcus aureus is one of the most important pathogens of human beings all over the world and all ages. The epidemiology and resistant pattern of this bacterium is not clear in Ilam hospitals, capital of Ilam province, Iran. The antimicrobial pattern of S. aureus strains isolated from health-care associated infections of different hospitalization wards including surgery and ICU have been investigated at the Ilam Hospitals. Bacteria isolated from different samples in the microbiology laboratory of Ilam University of Medical sciences.strains identified under standard techniques. Antibiotic susceptibility and Minimum Inhibitory Concentrations was done by E-test and routine antibiotics. A total of 30 S. aureus isolates were isolated from Urinary (12; 40%), wound (10; 33.3%) lung (5; 16.6%), and burn (3; 10%) infections. All of the isolates were susceptible to vancomycin and linezolide and 10% of strains were methicillin resistant. Rresistance percent for other drugs were as follow: piperacillin/tazobactam 4(13.3%), ceftriaxone 11(36.3%), amoxicillin 13(43.4%), amikacin 1(3.3%), ceftazidime 2(6.6%), tobramycin 3(9.9%), tetracycline 11(36.3%), gentamicin 3(9.9%). Five isolate having MICs≥1.5 µg/ml against vancomycin. Although about 10 % our isolates were resistant to methicillin, and 100% were susceptible to vancomycin and linezolide, but in some of the isolates we encountered a rise in MICs against vancomycin. This point emphasizes the implementation of related control measures in hospitals for further control of treatment resistant infections.

Keywords: Staphylococcus aureus, Antimicrobial Susceptibility, MRSA, Health-care-Associated Infections.

 

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